The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains

نویسندگان

  • Fariba Mirzaei Bavil Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Horeyeh Bagban Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Hossein Nazemiyeh Research Centre for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mehdy Ahmady Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • MohammadReza Alipour Tuberculosis and Lung Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
  • Rana Keyhanmanesh Tuberculosis and Lung Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده مقاله:

  Objective(s): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect of this plant on tracheal chains of guinea pigs. Therefore, for the first time, in the present study, in order to identify main constituents of the methanolic extract, the relaxant effects of five different methanolic fractions (20%, 40%, 60%, 80%, and 100%) of N. sativa   on tracheal chains of guinea pigs were examined.     Materials and Methods:     The relaxant effects of four cumulative concentrations of each fraction (0.8, 1.2, 1.6, and 2.0 g%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6, and 0.8 mM) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 μM methacholine (group 2).     Results: In group 1, all concentrations of only theophylline showed significant relaxant effects but all concentrations of these methanolic fractions showed significant contractile effects compared with that of saline (P

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منابع مشابه

The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains

OBJECTIVE(S) In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect...

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the relaxant effects of different methanolic fractions of nigella sativa on guinea pig tracheal chains

objective(s): in regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. our previous studies demonstrated the preventive effect of nigella sativa extract on ovalbumin-induced asthma. in addition, water-soluble substances of n. sativa extract and methanol fraction of this plant were responsible for the relaxant effec...

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Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism(s).

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the main relaxant constituents of nigella sativa methanolic fraction on guinea pig tracheal chains.

our  previous  studies demonstrated  the  preventive effect  of  nigella  sativa  extract  on asthma and water-soluble substances of this extract, especially its methanol fraction were responsible for this relaxation on contracted tracheal chains of guinea pigs. for the first time, the present study has been conducted to determine the main constituents of its methanolic extract. four constituen...

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Possible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains

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Possible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains

Achillea wilhelmsii have been used in folk remedies. The relaxant effects of the extractof A. wilhelmsii on tracheal chains of guinea pigs were examined. The relaxant effects offour cumulative concentrations of the extract, theophylline and saline were examined bytheir relaxant effects on precontracted tracheal chains of guinea pig by KCl (group 1), 10 μMmethacholine (group 2), incubated tissue...

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عنوان ژورنال

دوره 16  شماره 2

صفحات  123- 128

تاریخ انتشار 2013-02-01

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